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In anaerobic digestion (AD) of sewage sludge, elevated glucose supplementation has been shown to support the dominance of acetate-based methanogenesis, while lower glucose concentrations typically lead to reduced volumetric methane production but higher methane yield and enhanced CO₂ conversion efficiency. However, the combined influence of moderate glucose enrichment and gradual temperature elevation on methane production and microbial community dynamics remains insufficiently explored. This study aims to address that gap by examining the effects of moderate glucose addition (5 g/L) under a stepwise temperature transition from 37 °C to 45 °C in 2 °C increments. The results revealed a slight reduction in methane production at 39 °C and 41 °C, coupled with a transient increase in CO₂ output, most notably at 39 °C. However, methane generation improved significantly at 43 °C and 45 °C, reaching a maximum of 210 mL CH₄/g COD. Pearson correlation and non-metric multidimensional scaling (NMDS) analyses indicated that the increase in methane yield was strongly associated with the enrichment of hydrogenotrophic Methanoculleus, supported by syntrophic acetate-oxidizing bacteria (SAOB), namely Anaerolinea and Cloacamonaceae W22. These findings highlight the critical role of syntrophic partnerships in sustaining methanogenesis under elevated thermal conditions and offer key insights for improving biogas production efficiency through microbial community optimization.

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Syntrophic Microbial Adaptation Enhances Biogas Production During Temperature Transition to 45 °C with Moderate Glucose Supplementation

  • 会议论文

  • 发表日期：

    2026年

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  • Gede Adi Wiguna Sudiartha,
  • Tsuyoshi Imai,
  • I. Wayan Budiarsa Suyasa,
  • Hanna Anugerahani,
  • Nelson Darma Effendi,
  • Cokorda Istri Niti Laksmi Dewi,
  • Ni Komang Puspa Arjiani

摘要

In anaerobic digestion (AD) of sewage sludge, elevated glucose supplementation has been shown to support the dominance of acetate-based methanogenesis, while lower glucose concentrations typically lead to reduced volumetric methane production but higher methane yield and enhanced CO₂ conversion efficiency. However, the combined influence of moderate glucose enrichment and gradual temperature elevation on methane production and microbial community dynamics remains insufficiently explored. This study aims to address that gap by examining the effects of moderate glucose addition (5 g/L) under a stepwise temperature transition from 37 °C to 45 °C in 2 °C increments. The results revealed a slight reduction in methane production at 39 °C and 41 °C, coupled with a transient increase in CO₂ output, most notably at 39 °C. However, methane generation improved significantly at 43 °C and 45 °C, reaching a maximum of 210 mL CH₄/g COD. Pearson correlation and non-metric multidimensional scaling (NMDS) analyses indicated that the increase in methane yield was strongly associated with the enrichment of hydrogenotrophic Methanoculleus, supported by syntrophic acetate-oxidizing bacteria (SAOB), namely Anaerolinea and Cloacamonaceae W22. These findings highlight the critical role of syntrophic partnerships in sustaining methanogenesis under elevated thermal conditions and offer key insights for improving biogas production efficiency through microbial community optimization.