In a soil from Joya de los Sachas in eastern Ecuador, which has a Ci of 200 mg/kg of fluoranthene, it was remediated with Fe nanoparticles (0) synthesized with Drago’s blood (Croton lechleri) extract (nZVI-C.l.), and subsequently biostimulated. In the synthesis of nZVI-C.l., the extract of Drago’s blood acts as a reducing and stabilizing agent; the concentration of polyphenols was determined by the Folin-Ciocalteu method, and their antioxidant capacity by the DPPH free radical method. The NPs were characterized using DLS, UV-visible spectroscopy, XRD, and TEM. To determine the removal of fluoranthene, batch tests were performed by analyzing the initial and final concentrations using gas chromatography, along with their respective removal kinetics and subsequent biostimulation through nutrient addition. Twelve fluoranthene-degrading strains were identified during this study by amplification of the 16S ribosomal gene. The concentration of polyphenols in the extract was 52.852 (±4.68) mg gallic acid/L, accompanied by an antioxidant capacity of 87.54%. The inhibitory diameter of the nanoparticles in the cultures of the strains above varied between 7.53 ± (0.27) nm and 19.83 ± (0.86) nm, and the XRD spectrum showed peaks associated with zero-valent iron. By UV-visible spectrophotometry, the spectrum of the Drago’s blood extract was differentiated from the spectrum of the nZVI-C.l., observing a peak of absorbance of the extract corresponding to catechins and the decrease of absorbance of the nanoparticles after 24 h of being synthesized. Thus, achieving a contaminant removal of 43.67% using nZVI-C.l. and 69.18% with biostimulation.