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The current study involved the isolated 172 local yeast strains over a three-month period from November 1/2023 to February 1/2024 from various Fruit, Leaves and Soil, collected from home gardens and local markets in the Mosul city/Iraq. These strains were identified after cultivation on solid Malt Extract Agar (MEA) medium at the morphological and biochemical methods. The results showed that they belong to eight different species, which were confirmed with molecular identification by polymerase chain reaction (PCR) techniques, where the nucleotide sequence analysis of them were subsequently registered in the National Center of Biotechnology Information (NCBI) GenBank with the following names: Naganishia uzbekistanensis MAB1, Pichia nakasei MAB2, Moesziomyces aphidis MAB4, Pichia kudriavzevii MAB5, Hanseniaspora guilliermondii MAB6, Hanseniaspora uvarum MAB7, Rhodotorula mucilaginosa MAB8 and Meyerozyma guilliermondii MAB9.

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Isolation and Identification of Yeasts from Fruits, Leaves and Soil of Some Plants in the Mosul City

  • 会议论文

  • 发表日期：

    2026年

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  • Marwa Bader Falih Alobady,
  • Amjad Abdul-Hadi Mohammed,
  • Badia Abdul Razzaq Malla Obaida

摘要

The current study involved the isolated 172 local yeast strains over a three-month period from November 1/2023 to February 1/2024 from various Fruit, Leaves and Soil, collected from home gardens and local markets in the Mosul city/Iraq. These strains were identified after cultivation on solid Malt Extract Agar (MEA) medium at the morphological and biochemical methods. The results showed that they belong to eight different species, which were confirmed with molecular identification by polymerase chain reaction (PCR) techniques, where the nucleotide sequence analysis of them were subsequently registered in the National Center of Biotechnology Information (NCBI) GenBank with the following names: Naganishia uzbekistanensis MAB1, Pichia nakasei MAB2, Moesziomyces aphidis MAB4, Pichia kudriavzevii MAB5, Hanseniaspora guilliermondii MAB6, Hanseniaspora uvarum MAB7, Rhodotorula mucilaginosa MAB8 and Meyerozyma guilliermondii MAB9.