Colleges and universities are divided into basic laboratories and specialized laboratories, among which most specialized laboratories are set up according to their specialties and used for practical teaching of various specialties. Based on cloud technology, this paper makes a further study on the construction of university laboratory and management system. Through the analysis of experimental data, it can be found that the accuracy rate of the system designed in this paper is higher than that of the traditional system, and the highest accuracy rate of the system designed in this paper can reach 90.08%. However, the highest accuracy of the traditional system is only 61.23%. Through the above experimental data, it can be found that the system designed in this paper is generally higher than the traditional system, both in terms of reading and writing data test speed and reading and writing data test accuracy. The design of this system is based on the above functional requirements. On the basis of realizing the functions of system user information management, laboratory course information management, discipline construction management and experimental project management, cloud computing is introduced to improve the speed of system management information management and file storage capacity.