This chapter provides a detailed summary of the essential physical and operational parameters associated with commonly utilized therapeutic lasers in ophthalmology. It emphasizes the importance of understanding each laser’s application-specific role, including its wavelength, pulse duration, energy output, and tissue absorption characteristics. These biophysical properties are critical for tailoring laser treatments to specific ocular conditions such as glaucoma, diabetic retinopathy, or refractive errors. However, it is important to note that actual clinical implementation may vary based on manufacturer specifications, device calibration, and individual patient factors such as ocular anatomy and disease severity. This variability underscores the need for personalized treatment planning and ongoing evaluation of laser efficacy and safety in practice.