Practical training is essential in health education, but real-life practice is often limited by high costs, safety risks, and resource constraints. Virtual Reality (VR) offers a safe, visual, and flexible alternative that allows students to perform experiments without direct exposure to hazardous chemicals. It also reduces instructors’ preparation time and ensures consistent learning experiences across student groups. This study develops a VR system and explores the potential of VR technology as an innovative solution to overcome these challenges, aiming to improve students’ practical competencies while reducing associated costs and risks. Additionally, the system’s tracking capabilities enhance learning outcomes by providing detailed performance feedback. An experiment was conducted using a practical exercise on Thin Layer Chromatography (TLC) at Hanoi Medical College to design and evaluate the VR-based training application. The study also investigates learners’ perceptions and discusses the effectiveness, feasibility, and scalability of VR integration in health education, proposing future directions for further development and broader implementation of this technology.