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Genome editing offers unprecedented opportunities for developing crops with enhanced traits, including improved yield, disease resistance, drought tolerance, and nutritional content. CRISPR/Cas9 and TALEN are most commonly used systems for site-directed mutagenesis. CRISPR/Cas9, a system derived from bacterial defense mechanisms, provides a highly efficient, targeted approach for gene editing that resulting from single-base pair changes or larger structural modifications in plant genomes. On the other hand, TALENs, which utilize custom-designed DNA-binding proteins, offer an alternative method for achieving similar precision in gene modification. This chapter provides a comprehensive overview of the principles, mechanisms, and applications of CRISPR/Cas9 and TALEN in crop improvement. Additionally we also discuss the comparative advantages and limitations of each system, including their efficiency, specificity, ease of use and the regulatory and ethical concerns associated with genome-edited crops.

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CRISPR/Cas9 and TALEN-Mediated Genome Engineering for Crop Improvement

  • 章节

  • 发表日期：

    2026年

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  • Nusrat Perveen,
  • Javid Iqbal Mir,
  • Hidayatullah Mir

摘要

Genome editing offers unprecedented opportunities for developing crops with enhanced traits, including improved yield, disease resistance, drought tolerance, and nutritional content. CRISPR/Cas9 and TALEN are most commonly used systems for site-directed mutagenesis. CRISPR/Cas9, a system derived from bacterial defense mechanisms, provides a highly efficient, targeted approach for gene editing that resulting from single-base pair changes or larger structural modifications in plant genomes. On the other hand, TALENs, which utilize custom-designed DNA-binding proteins, offer an alternative method for achieving similar precision in gene modification. This chapter provides a comprehensive overview of the principles, mechanisms, and applications of CRISPR/Cas9 and TALEN in crop improvement. Additionally we also discuss the comparative advantages and limitations of each system, including their efficiency, specificity, ease of use and the regulatory and ethical concerns associated with genome-edited crops.