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Lipases are enzymes that can catalyze both the breakdown and formation of fats and can be extracted from various organisms. Microorganisms, such as bacteria, fungi, and yeasts, through microbial fermentation, offer an ecological alternative for producing lipases, thereby favoring the sustainable development of various industrial applications, ranging from the food and pharmaceutical industries to waste management. However, significant challenges still need to be overcome, such as reducing production costs and overcoming technical barriers to achieve industrial scalability. This work reviews recent advances in lipase production using solid-state fermentation (SSF) and submerged fermentation (SmF) as effective methods for large-scale industrial lipase production developed through enzyme engineering techniques. Additionally, the stability of lipases under extreme conditions and the importance of advanced techniques to improve catalytic efficiency are discussed. Despite immediate challenges, the results show that microbial lipases are essential for achieving more sustainable and environmentally friendly industrial processes.

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Microbial Fermentation for Lipase Production: Advances in Enzyme Engineering and Industrial Applications

  • 章节

  • 发表日期：

    2026年

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  • Rodrigo da Silva Vieira,
  • Francisca Tayane de Souza Amorim,
  • Francisco Izaias da Silva Aires,
  • Larysse Maria Santiago de Castro,
  • Patrick da Silva Sousa,
  • Iury Barbosa Pereira,
  • Maurício Quintas Salamba,
  • Dayana Nascimento Dari,
  • José Cleiton Sousa dos Santos

摘要

Lipases are enzymes that can catalyze both the breakdown and formation of fats and can be extracted from various organisms. Microorganisms, such as bacteria, fungi, and yeasts, through microbial fermentation, offer an ecological alternative for producing lipases, thereby favoring the sustainable development of various industrial applications, ranging from the food and pharmaceutical industries to waste management. However, significant challenges still need to be overcome, such as reducing production costs and overcoming technical barriers to achieve industrial scalability. This work reviews recent advances in lipase production using solid-state fermentation (SSF) and submerged fermentation (SmF) as effective methods for large-scale industrial lipase production developed through enzyme engineering techniques. Additionally, the stability of lipases under extreme conditions and the importance of advanced techniques to improve catalytic efficiency are discussed. Despite immediate challenges, the results show that microbial lipases are essential for achieving more sustainable and environmentally friendly industrial processes.