This study was conducted to assess the traditional plant-based viz. ethno-botanical remediesEthno-botanical remedies used by pastoralists of the CholistanCholistan Desert in Pakistan for the control and treatment of livestock diseases and ailments. Across five locations we used a structured questionnaire to collect data from 100 livestock farmers (LF) and 20 livestock healersLivestock healers (LH). From correlation analyses the three least correlated variables were identified among five, which were representative of LFs. A cluster analysis was performed on the basis of these three variables and LFs were grouped into three logically different clusters. Kruskal–Wallis tests and crosstab analyses were used to detect significant differences between clusters and effects of various variables on their use of ethno-botanical remediesEthno-botanical remedies. Most of the male only respondents (LF 78%; LH 70%) were married and illiterate (LF 66%; LH 70%). LH had larger herds (average 109 animals) than LF (average 85 animals) and were more experienced in livestock husbandry and management. LF spent about 162.5 Euros annually on the treatment of their livestock, but there was great variability in expenditures. Average animal treatment experience of LH was 29 years; all were experts in treatment of all types of diseases (100%) and animal species (70%). Eighty-six traditional remedies based on 64 plants belonging to 43 families were used. Capparaceae was the botanical family with the largest number of used species (4), followed by Chenopodiaceae, Poaceae, Solanaceae and Zygophyllaceae (3). Aerial parts (43%), leaves (26%), fruits (9%), seeds and seed oils (9%) were frequently used parts, while flowers, roots, bulbs and pods were less frequently employed (<5%). Common preparations were decoction, jaggery and ball drench; oral drug administration was very common and doses were estimated using lids, spoons, cups and handfuls. Doses used for different animal species varied depending on animal age, size and physical condition and severity of the disease. Pastoralists are practicing traditional plant-based livestock medication without scientific validation as they cannot afford allopathic drugs due to their livelihood conditions. Therefore, the efficacy of documented medicinal plantsMedicinal plants against the most prevalent livestock diseases should be evaluated in order to recommend effective preparations and treatments to this poor population group.