The emergence of Industry 5.0, following the widespread adoption of Industry 4.0, marks a pivotal shift in digitalization and industrial operations. This article explores the implications of Industry 5.0 principles and enabling technologies within the Architecture, Engineering, Construction, Management, Operation, and Conservation (AECMO&C) industry, with a particular focus on the conservation of built cultural heritage environments. The results obtained from a systematic literature review and an online survey are summarized and discussed. Results reveal that artificial intelligence and digital twins are the most frequently studied enabling technologies in this context, while sustainability emerges as the dominant principle in the discourse surrounding this novel paradigm. Conversely, the principles of resilience and human-centrism remain underexplored, highlighting the need for further research to achieve a holistic implementation of Industry 5.0 in conservation practices. Furthermore, although awareness of Industry 5.0’s potential is growing, its adoption in heritage conservation remains limited due to knowledge gaps, inadequate training, and resource constraints. This underscores the need for comprehensive strategies to integrate Industry 5.0 principles and technologies into the conservation of built cultural heritage. Insights presented are intended to guide conservation practitioners seeking best practices, inform policymakers promoting technological adoption, and inspire researchers to address existing gaps and drive further innovation.