The prevalence of light pollution, particularly stemming from road infrastructure, poses a substantial environmental concern necessitating sustained investigation. Emphasizing the criticality of preserving natural landscapes, especially within protected areas, where ecosystems are notably vulnerable, this chapter unveils the consequential effects of road lighting on biodiversity and elucidates the efficacy of integrating satellite remote sensing and traffic data to comprehensively understand and mitigate the multifaceted impacts on both biodiversity and human structures. Leveraging advancements in remote sensing technologies, this research advocates for judicious road lighting adaptations and strategic traffic policies to minimize wildlife endangerment. Furthermore, through environmental impact assessments and computational models simulating diverse lighting scenarios, proactive measures are recommended to preclude adverse effects preemptively. This proactive stance underscores the imperative for a harmonious balance between human infrastructural needs and ecological conservation. This interdisciplinary pursuit, integrating advanced technologies, rigorous research, and informed policy implementations, stands pivotal in curbing infrastructural-induced light pollution, ensuring the preservation of biodiversity for future generations.