Transportation infrastructure is essential for modern society, but its expansion poses a threat to protected and conserved areas and the ecosystems and biodiversity they harbor. With greater attention to this dilemma—and more solutions available than ever—there is still a need for alignment and collaboration among countries, sectors, and disciplines: government, private sector, NGOs, academia, and other partners. The world’s foremost nature conservation organization, the International Union for the Conservation of Nature (IUCN), is well-positioned to bring together these entities and is doing so through the Transport Working Group (TWG), a network of voluntary members hosted by the IUCN World Commission on Protected Areas and its Connectivity Conservation Specialist Group. In its formative years, the TWG has built a robust membership of transportation ecologists and allied practitioners globally, strengthened communication channels across continents, and coordinated the sharing of resources and expertise. Species-specific and geography-specific subgroups have also been formed to test whether context-sensitive efforts can more effectively support the TWG’s goals. Technical publications with subsequent capacity-building and practical implementation are now expanding TWG’s influence and role as a leading international network of practitioners focused on transportation ecology.