The purpose of this chapter is to define the importance and determinants of employee well-being, with particular emphasis on the importance of organizational culture and its impact on employee well-being in hybrid work environments. The essence of well-being and the importance of organizational culture in the context of employee job satisfaction are presented. The discussion focuses on the relationships between the following categories: employee well-being, organizational culture, and hybrid work. Employee well-being is a crucial aspect of life in the workplace. Employee well-being influences not only employees’ well-being and quality of life but also the effectiveness and efficiency of the organization. It is generally accepted that a proper organizational culture has a positive impact on interpersonal relationships, communication, development, and teamwork, which should also translate into job satisfaction, i.e., employee well-being. With this assumption in mind, an attempt was made to determine the relationships between these phenomena in a given hybrid work environment. An attempt was made to assess managers’ awareness of these relationships, considering their role in shaping organizational culture and their impact on employee well-being. The method employed was an analysis of management literature and qualitative and quantitative research methods.