E-commerce is a fast-growing industry, yet it is struggling to address the sustainable impact of its supply chain. In this context, the last mile—the transportation of online orders from the e-retailer’s last point of contact with the package to the point of consumption—emerges as the main contributor to this impact. To manage this effect and provide faster deliveries, e-retailers are incorporating sustainable and resilient practices such as dark stores. Dark stores are small facilities that serve as logistical hubs for product storage, online order fulfillment, and delivery. They are typically located in or near the delivery zone and contain the inventory used to fulfill all orders within the delivery zone. Following the introduction of dark stores, researchers have expressed interest in this innovation. However, the literature is scattered and focuses on different aspects, which makes it difficult to determine whether dark stores are a suitable solution to reduce the impact on sustainability of e-commerce. In this context, the aim of this research is to structure the knowledge on dark stores in e-commerce through the creation of a conceptual framework. In this way, current knowledge is organized to establish a conceptual framework. This framework integrates knowledge about when and how dark stores should be implemented and their impact on sustainability. Furthermore, a panel of experts is consulted to evaluate the correctness and usefulness of this conceptual framework. In addition to the construction of the conceptual framework, this paper also identifies the existing gaps in the literature on this resilient practice.