One common multidrug-resistant (MDR) bacterium that can cause a variety of illnesses in hospitalized patients is Klebsiella pneumoniae, sometimes known as K. pneumoniae. With the increasing use of antibiotics, MDR K. pneumoniae (MDR-KP) is becoming more common, posing additional difficulties and obstacles for clinical treatment. Therefore, the purpose of this study was to evaluate the efficacy of Artemisia extract and cefotaxime antibiotic against Klebsiella pneumoniae. Twenty-five clinical isolates of bacteria from Prince Hamza Hospital, were collected. To examine their impact on bacteria, the combination assay and disc diffusion were employed. The CTX minimum inhibitory concentration (MIC) was ascertained both prior to and after to its combination with the Artemisia extract. The study found that combining Artemisia herba-alba with cefotaxime significantly enhanced the antibacterial activity against Klebsiella pneumoniae. Micro-dilution broth tests revealed that when cefotaxime was used alone, the minimum inhibitory concentration (MIC) ranged from 4 to 8 mg/L, with some samples exhibiting complete resistance. However, after the introduction of Artemisia herba-alba extract, the MIC values increased to between 25 and 100 mg/L for all samples. Remarkably, the combination of cefotaxime and Artemisia herba-alba extract led to a significant reduction in the MIC for cefotaxime, which ranged from 0.125 to 0.064 mg/L across all samples. This study emphasizes how crucial it is to look into natural substances that may be able to limit antibiotic resistance and These findings support the need for further investigation into the potential of combination therapy for combating Klebsiella pneumoniae infections. Additional studies are required to fully understand the efficacy of these treatments against this pathogen.