Objective: The study aims to examine the effect of thyroid dysfunction on lipid profile in the Jordan community. Method: This is a retrospective study. The study includes randomly selected 430 participants. Collected data were analyzed using the SPSS program. The results of thyroid stimulation hormone were compared with lipid profile components (cholesterol total serum (CHT), low-density lipoproteins (LDL), high-density lipoproteins (HDL), triglycerides TRG (TRG), cholesterol/HDL ratio (C/HDL), and non-cholesterol HDL (NHDLC)). Results: There is an abnormal increase in the results of the variables (Cholesterol Total, Cholesterol/HDL Ratio, HDL Cholesterol) and variable (Thyroid Stimulating Hormone (TSH)), where the correlation coefficients were positive and statistically significant. There is an inverse relationship with statistical significance at the significance level (p ≤ 0.05) between variables (Triglycerides, Non-HDL Cholesterol) and variable TSH, where the correlation coefficients were negative and statistically significant. Conclusion: There is a significant relationship between the elevated TSH readings and the readings of lipid profiles in patients with hyperthyroidism patients in Jordan.