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The PAO1 gene is one of the most important contributing factors among the genes that promote antimicrobial resistance in Pseudomonas Aeruginosa. The current study is comprehensive experimental was aimed to evaluate the prevalence of PAO1 gene in clinical isolates of Pseudomonas Aeruginosa, and its association with Artemisia Herba Alba Extractextractand Cefotaxime. The method use 30 clinical bacterial isolates of MDR and intermediate resistance Pseudomonas Aeruginosa were selected for the study and were fully characterized. Polymerase Chain Reaction (PCR) was used to evaluate the presence of PAO1 gene in the test subjects. The test subjects were grouped into four treatment types: Bacterial (control), Cefotaxime treatment, Cefotaxime and Artemisia Herba Alba Extract, and Artemisia Herba Alba Extract only. The genotypes of PAO1 were studied using minimum inhibitory concentrations (MIC), maximal efficacy against the chosen treatment groups, and polymorphisms present in the strains treated with various treatments were ascertained. The result showed that; As per the genetic analysis, the PAO1 gene was located in 90% of the studied isolates especially in all groups of treatment. This high prevalence suggests a significant genetic predisposition towards antibiotic resistance in the Pseudomonas Aeruginosa strains that were included in this study. Interestingly, the combination of Cefotaxime with Artemisia Herba Alba Extract showed a synergism effect in 90% of the isolates. In conclusion the PAO1 gene was available and widespread in Pseudomonas Aeruginosa isolates in this clinical study and therefore the presence of PAO1 makes the treatment of bacterial strains with intrinsic resistant mechanisms difficult. The molecular approach utilized in this study provides valuable insights into the synergistic interaction between Artemisia herba alba extract and Cefotaxime against P. aeruginosa. This paves the way for the development of novel combination therapies to address the growing threat of antibiotic resistance in healthcare settings.

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The Impact of (PAO1) Antimicrobial Resistance Gene in Pseudomonas Aeruginosa and its Inhibition with Cefotaxime and Artemisia Herba Alba Extract

  • 章节

  • 发表日期：

    2026年

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  • Hashem Abu Harirah,
  • Assia Hassan Ben Braiek,
  • Ali Mohammed Hassan,
  • Sami Ahmed Zaher Basha,
  • Ahmad Mohammad Al-Adabaiebah,
  • Alaa Adel Jiarah,
  • Andrew Atoom,
  • Nader Ahmed Jaradat

摘要

The PAO1 gene is one of the most important contributing factors among the genes that promote antimicrobial resistance in Pseudomonas Aeruginosa. The current study is comprehensive experimental was aimed to evaluate the prevalence of PAO1 gene in clinical isolates of Pseudomonas Aeruginosa, and its association with Artemisia Herba Alba Extractextractand Cefotaxime. The method use 30 clinical bacterial isolates of MDR and intermediate resistance Pseudomonas Aeruginosa were selected for the study and were fully characterized. Polymerase Chain Reaction (PCR) was used to evaluate the presence of PAO1 gene in the test subjects. The test subjects were grouped into four treatment types: Bacterial (control), Cefotaxime treatment, Cefotaxime and Artemisia Herba Alba Extract, and Artemisia Herba Alba Extract only. The genotypes of PAO1 were studied using minimum inhibitory concentrations (MIC), maximal efficacy against the chosen treatment groups, and polymorphisms present in the strains treated with various treatments were ascertained. The result showed that; As per the genetic analysis, the PAO1 gene was located in 90% of the studied isolates especially in all groups of treatment. This high prevalence suggests a significant genetic predisposition towards antibiotic resistance in the Pseudomonas Aeruginosa strains that were included in this study. Interestingly, the combination of Cefotaxime with Artemisia Herba Alba Extract showed a synergism effect in 90% of the isolates. In conclusion the PAO1 gene was available and widespread in Pseudomonas Aeruginosa isolates in this clinical study and therefore the presence of PAO1 makes the treatment of bacterial strains with intrinsic resistant mechanisms difficult. The molecular approach utilized in this study provides valuable insights into the synergistic interaction between Artemisia herba alba extract and Cefotaxime against P. aeruginosa. This paves the way for the development of novel combination therapies to address the growing threat of antibiotic resistance in healthcare settings.