This chapter delves into the evolution of dairy regulations in Africa, focusing on their development, implementation, and impact on the dairy industry. It begins with an introduction to early regulations, outlining their initial purposes and objectives. The progression of these regulations over time is analyzed, highlighting shifts in policies and practices to adapt to the growing demands of the dairy sector. Key aspects of milk production and transportation are explored, emphasizing the importance of maintaining quality and safety throughout the supply chain. The role of the Dairy Industry Act license is discussed, showcasing its significance in regulating market entry and operations. The chapter also examines regulatory barriers that affect trade within Africa, underscoring the need for harmonized standards to boost regional and international competitiveness. The discussion extends to sampling, testing, and grading practices, including their application in milk collection, distribution, and processing. A critical assessment is made of dairy safety, antimicrobial and drug residue monitoring, and overall milk safety standards. Consumer protection and labeling regulations are also addressed, with a focus on ensuring transparency, quality control, and safeguarding public health. The chapter concludes by summarizing the state of dairy regulation, emphasizing the importance of robust regulatory frameworks in promoting a safe, efficient, and competitive dairy industry in Africa.