The striatin-interacting phosphatase and kinase (STRIPAK) complex plays a pivotal role in the regulation of numerous developmental processes in fungi. The complex is a special variant of phosphatase 2A (PP2A), a highly conserved serine-threonine phosphatase composed of catalytic subunits (PP2Ac), a scaffolding subunit (PP2Aa), and various substrate-directing B regulatory subunits. Of particular interest in the STRIPAK complexSTRIPAK complex is the B”’ regulatory subunit, also known as striatinstriatin. STRIPAK is a signaling hub integrating kinases and upstream and downstream information from many conserved eukaryotic signaling pathways and has been the focus of significant research over the past two decades. Studies in fungi have significantly contributed to our current understanding of STRIPAK assembly, composition, and cellular localization. These studies also revealed its critical regulatory role in fungal development and pathogenicity. A comprehensive understanding of STRIPAK’s regulatory role paves the way to unraveling its multifaceted functions.