Hyphae of filamentous fungi extend at the apex by a polarized mechanism that involves the highly ordered and regulated delivery of secretory vesicles. In Dikarya fungi, these tip-directed vesicles accumulate temporarily at the Spitzenkörper (SPK) before being delivered to the apical plasma membrane, where they fuse and provide the materials needed for cell wall synthesis and membrane expansion. The organizational complexity of the SPK has been characterized in only a few fungal taxa, and it remains unclear whether the observed structural differences correspond to distinct functional roles. Hyphal tip growth also requires coordinated interactions with the cytoskeleton for precise positioning of the SPK and key secretory organelles (e.g., Golgi, endoplasmic reticulum), as well as the targeted delivery of secretory vesicles for sustained hyphal elongation.