The study shows the possibility of using the technology of biomineralization of cement composites using enzymes of urease of microorganisms and plant raw materials capable of forming mineral deposits. The obtained urease enzyme from bacterial and plant biomass samples was immobilized by the “inclusion in gel” method using sodium alginate. The activity of urease was evaluated and found to be in the range of 42–104 mg NH4+/g/day. The best results were shown by samples of urease from plant raw materials; however, the complexity and extremely low yield during isolation will not allow their rational use for cement composites. It is optimal to use a dietary supplement based on soil bacteria of the bacillus family, the activity of which was 95 mg NH4+/g/day. Concrete mixes were made using Portland cement CEM I 42.5 N. Modified cement composites with urease bioadditives have been studied using methods such as AFM, SEM and XFA. The time period during which new formations are formed in the pores of concrete using AFM has been studied. The use of XFA made it possible to determine the nature of the formed mineral sediments with the formula CaCO3 corresponding to the calcite mineral. According to the results of physico-mechanical tests, it was found that compressive strength and frost resistance increase compared to control samples that do not contain stripped-down dietary supplements. The maximum strength was shown by a sample with a dietary supplement content of 5% and at W:С = 0.5. Compressive strength increased by an average of 30–40% compared to the control experiment. The frost resistance of the modified samples increased to values of F1 - 550 cycles.