Nanoformulations, with particle sizes ranging from 10 to 100 nm, were employed to enhance drug delivery by improving solubility, stability, and enabling controlled release. These systems protected therapeutic agents from environmental degradation and facilitated targeted delivery, thereby optimizing therapeutic outcomes with lower dosing requirements. Deep eutectic solvents (DESs) have gained attention as eco-friendly substitutes for traditional solvents in the synthesis of nanomaterials. Owing to their low toxicity, biodegradability, and versatility, DESs enhanced the solubility, stability, and reactivity of nanoparticles, allowing precise control over nanoparticle formation. In this chapter, the use of DESs in synthesizing carbon-based, polymeric, and metal-based nanomaterials was discussed, demonstrating their wide-ranging potential in areas such as biomedicine, catalysis, energy storage, and environmental remediation. The integration of DESs with nanotechnology fostered sustainable innovation, paving the way for cleaner and more efficient advancements in materials science.