In recent years, electrochemical research has significantly advanced in the detection of substances critical to human health, with amino acidsAmino acids being a focal point due to their essential role in numerous biological processes. Imbalances in amino acid levels often serve as indicators of various diseases. In the context of environmental science, the detection of amino acidsAmino acids is also crucial for monitoring pollution, particularly in water bodies, where amino acid imbalances can signal contamination from industrial effluents or wastewater. This underscores the growing need for effective and reliable detection methods. Electrochemical technologies have demonstrated considerable promise for detecting electroactive amino acidsAmino acids like cysteine, tyrosine, tryptophan, methionine, and histidine. However, the detection ofNon-electroactive amino acids non-electroactive amino acidsAmino acids presents unique challenges because they lack intrinsic redox activity. This review focuses on recent advancements in electrochemical strategies for detectingNon-electroactive amino acids non-electroactive amino acidsAmino acids such as glycine, alanine, serine, and threonine, which are of interest in environmental monitoringEnvironmental monitoring. Key approaches, including enzyme-based sensors, nanomaterial-modified electrodesModified electrodes, and indirect electrochemical methods such as molecular imprinting, are discussed. Additionally, the challenges and future directions in expanding the scope of electrochemical detectionElectrochemical detection for these non-traditional electroactive amino acidsAmino acids are explored, with an emphasis on their potential for monitoring water qualityWater quality and environmental pollution. Environmental benefits such as cost-effectiveness, low energy consumption, and high selectivity of these detection methods are also highlighted, underscoring their relevance to sustainable environmental practices.