Textile finishing processes are responsible for clean water pollution due to the fossil-based finishing agents conventionally used. Agro-industrial wastes are valuable sources of bio-active compounds with interesting properties to develop functional textiles. In this study, in an aqueous process, the combination with enzymes were explored for functional compounds extraction. Hot trub (HT), extracted olive pomace (OPe) and oregano pruning stems (OPS) were selected for this study aiming to incorporate antioxidant, antimicrobial, UV protection and water repellence properties into textiles. The highest antioxidant activity was recorded on the textiles functionalized with HT hydroethanolic extract (86.68 ± 0.11%). The fabrics treated with OPS aqueous extract by impregnation (78.97 ± 4.10%) and exhaustion (73.22 ± 7.79%) show higher antioxidant properties compared to enzymatic extract. A superhydrophobic textile surface was developed impregnating HT hydroethanolic extract, with a water contact angle (WCA) of 158.98 ± 9.97. Hydrophobic textiles were developed by the impregnation with HT aqueous extract supplemented with enzymes, achieving a water contact angle of 140.60 ± 6.23° (cellulase and pectinase) and 132 ± 6.81° (cellulase). An excellent UV protection (UPF = 50/50+) was recorded on fabrics impregnated with aqueous extract of HT and OPS and a good protection (UPF = 30) with OPe, following exhaustion method. HT aqueous extracts show antimicrobial activity against S. aureus. In this study, multifunctional fabrics were developed using water-based sustainable methods and easily implemented in textile finishing industries facilities.