Kinetic Analysis of the Anaerobic Digestion of Horse Manure with Effluents from Pig Farms and Wastewater Treatment Plants
摘要
The agricultural sector, particularly intensive livestock systems, significantly contributes to environmental issues such as greenhouse gas (GHG) emissions and groundwater pollution. In this work effluents from pig farms and wastewater treatment plants (WWTPs) were used as a substrate and collected from a wastewater treatment plant in Alto Alentejo and a pig farm located in Baixo Alentejo, Portugal. Fresh horse manure was used as an inoculum in this study and was collected at a riding school in Alto Alentejo. Anaerobic digestion tests were performed in 1000 mL glass reactors with a 700 mL working volume, incubated at 38 ± 1 ℃ for 28 days under mesophilic conditions. Reactors were loaded with effluent and inoculum at a 1:9 inoculum-to-substrate mass ratio. The composition and volume of biogas were analyzed weekly. The maximum biogas yield achieved was 360 mL and 1730 mL, respectively, for pig effluent without and with horse manure inoculum. In the case of effluent from the WWTPs without and with the inoculum, the maximum biogas achieved was 980 mL and 1220 mL, respectively. The biogas production was optimized, predicted, and simulated under various conditions using mathematical kinetic models. Three mathematical kinetic models—the first-order kinetic model, the modified Gompertz model, and the logistic model—were applied to estimate key parameters, including biogas production potential, maximum production rate, and lag time, by fitting the observed biogas yields. The modified Gompertz model is widely regarded as a reliable empirical non-linear regression model for predicting methane accumulation. It provides crucial information on the lag phase and the maximum specific methane production rate, characterizing microbial growth in terms of exponential growth rates and lag phase duration. The modified Gompertz model predicted a production of 980 mL and 1276 mL for WWTP effluent with and without inoculum, respectively, and for pig effluent with and without inoculum, it predicted a production of 1743 mL and 369 mL, respectively.