Building on the growing interest in adaptive game design and affective gaming, this research integrates psychometric assessments, cognitive control tasks, perceptual modality evaluations, and real-time eye-tracking within a unified experimental framework. Twenty participants (aged 18–39) completed the Five-Factor Personality Inventory (FFPI), 2-back working-memory and Stroop tasks, perceptual modality test (S. Efremtsev), and a 15-min gaming session in Doom (2016) while eye movements were recorded with Pupil Labs eye-tracking glasses. The results demonstrate several significant associations. Conscientiousness showed positive correlations with fixation count (r = 0.47, p < 0.05) and fixation duration count (r = 0.45, p < 0.05), negative correlations with mean fixation duration (r = –0.44, p < 0.05) and cognitive fixations (r = –0.48, p < 0.05). Openness to experience was significantly associated with enhanced working-memory performance, as indicated by its negative correlation with image 2-back error rates (r = –0.46, p < 0.05). Moreover, openness correlated positively with both auditory (r = 0.49, p < 0.05) and kinesthetic modality preferences (r = 0.48, p < 0.05). Neuroticism demonstrated a positive correlation with visual modality preference (r = 0.45, p < 0.05). These findings highlight the relevance of psychological traits and perceptual preferences in shaping cognitive load, attentional deployment, and gaze dynamics during interactive gameplay. The results provide a foundation for future research on adaptive biofeedback systems that integrate personality profiling and real-time biometric monitoring to optimize game-based learning, therapeutic interventions, and esports training.