In recent years, the issue of early-onset visual impairment in children has become increasingly severe. As an important space for preschool children’s extended activity and learning, the natural lighting design of kindergartens plays a vital role in safeguarding children's visual health. The paper uses the Grasshopper genetic algorithm optimization tool Wallacei plugin, takes a kindergarten in Zhengzhou as an example, and uses a parameterized method to control the parameters of window-to-wall ratio, window sill height, window height and interfenestration, with aDA, aUDI, and aDGP as the evaluation indexes to optimize the natural lighting design in the kindergarten living unit. Simultaneously, correlation and linear regression analyses are conducted on the simulated samples. The findings indicate that: (1) The independent variables window-to-wall ratio, window sill height, window height and interfenestration are linearly correlated with aDA, aUDI, and aDGP. (2) The aDA, aUDI, and aDGP of the kindergarten living unit can be increased by 6.47%, 3.1%, and 0.13, respectively.