Electrochemiluminescence (ECL) is a luminous phenomenon triggered by electrochemical reactions. Owing to its high sensitivity, low background interference, and excellent spatiotemporal control, ECL has become as a powerful analytical technique across diverse fields. In the past two decades, the integration of ECL with microscopy has emerged as a significant advancement, enabling single-molecule/entity imaging, high-throughput analysis, and electrocatalysis studies. This chapter provides essential knowledge for conducting ECL microscopy experiments. The core apparatus is firstly introduced, typically a custom setup integrating an optical microscope, detector, electrochemical cell, and workstation. Then, critical components that determine the experimental performance are summarized, including working electrode materials and types, ECL reagents, and electrochemical methods. Furthermore, ECL imaging modes and their corresponding application scenarios are discussed. Finally, the chapter addresses the challenges and future perspectives in ECL imaging.