Scholarlink

In this work, we present a murine small intestine bioreactor system composed of two hydraulic chambers that recreate nutrient exchange in the intestinal tissue walls and their respective microfluidic interactions. A custom-engineered microfluidic bioreactor with a dual-inlet/double-outlet configuration and peristaltic flow, featuring adjustable speed variations for luminal flow rate control, was developed. The model’s design was executed in SolidWorks and its fluid dynamics simulated in COSMOS Floworks, utilizing the universal physical properties of water, specifically an isothermal Newtonian liquid with a density of 1000 kg/m3, a viscosity of 0.889 mPa, a continuous flow rate of 0.015 m/s, and atmospheric pressure. The system’s construction material is black photocurable resin (Anycubic), characterized by an elastic modulus (E) of 1.2 GPa and a Poisson’s ratio of 0.3. This system effectively facilitates nutrient transport within a small intestine tissue model by mediating substance transfer between its chambers. Critically, this microfluidic platform accurately mimics murine small intestine tissue; the optimal design simulation demonstrates a flow rate of 20.36 nL/s and a minimal pressure drop of 107.32 mPa. This represents a significant advancement over previous in vitro murine models, mitigating prior limitations and accuracy concerns, thereby offering a more physiologically representative simulation of intestinal functions. The aim of this work is to obtain, through computational simulations, the ideal parameters for testing the microfluidic system with the media and parameters derived from the simulation.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Design and Development of a Small-Intestine-Chip System to Generate Microphysiologic Mourine Models

  • 会议论文

  • 发表日期：

    2026年

查看全文
  • O. Ramirez-Fernandez,
  • R. Legorreta-Atienzo,
  • F. Equihua-Guillen,
  • L. Castruita-Avila,
  • E. Camporredondo,
  • A. Garcia-Lara,
  • E. Zuñiga-Aguilar

摘要

In this work, we present a murine small intestine bioreactor system composed of two hydraulic chambers that recreate nutrient exchange in the intestinal tissue walls and their respective microfluidic interactions. A custom-engineered microfluidic bioreactor with a dual-inlet/double-outlet configuration and peristaltic flow, featuring adjustable speed variations for luminal flow rate control, was developed. The model’s design was executed in SolidWorks and its fluid dynamics simulated in COSMOS Floworks, utilizing the universal physical properties of water, specifically an isothermal Newtonian liquid with a density of 1000 kg/m3, a viscosity of 0.889 mPa, a continuous flow rate of 0.015 m/s, and atmospheric pressure. The system’s construction material is black photocurable resin (Anycubic), characterized by an elastic modulus (E) of 1.2 GPa and a Poisson’s ratio of 0.3. This system effectively facilitates nutrient transport within a small intestine tissue model by mediating substance transfer between its chambers. Critically, this microfluidic platform accurately mimics murine small intestine tissue; the optimal design simulation demonstrates a flow rate of 20.36 nL/s and a minimal pressure drop of 107.32 mPa. This represents a significant advancement over previous in vitro murine models, mitigating prior limitations and accuracy concerns, thereby offering a more physiologically representative simulation of intestinal functions. The aim of this work is to obtain, through computational simulations, the ideal parameters for testing the microfluidic system with the media and parameters derived from the simulation.