Surface functionalization of titanium alloys with TiO2 nanotubes has shown promise in biomedical applications due to their nanostructured morphology. In this study, bioactive coatings containing calcium-, phosphorus-, potassium-, magnesium-, and zinc-based elements were electrodeposited onto anodized TiO2 nanotubes formed on a Ti-407, an emerging titanium alloy with biomedical potential. Anodization was performed at 50 V, followed by electrodeposition at 5 V and 1 A in a mineral electrolyte. The resulting surfaces were characterized using SEM-EDS, revealing a uniform granular coating with high elemental incorporation. In vitro cell viability was assessed using the MTT assay with MG-63 osteoblast-like cells. The coated samples demonstrated significantly higher cell viability (112.5%) compared to uncoated controls. These findings suggest that the presence of the mineral-based bioactive coating enhances the cellular response under in vitro conditions. This work provides preliminary evidence supporting the potential of electrodeposited coatings on nanostructured titanium surfaces to improve osteoblast viability, offering a promising route for future surface modification strategies in implantable biomaterials.