The present research examines Templar Church of Saint Bevignate (Perugia, Italy) structural analysis, considering targeted structural interventions since the 1990s. These efforts were aimed at mitigating the seismic vulnerability of the building, without compromising its valuable Templar frescoes, which represent a significant part of site’s cultural and artistic heritage. The seismic behaviour of San Bevignate has been investigated through a range of global analytical approaches performed using an equivalent frame model. These include a linear static analysis to assess static performance, and non-linear static analysis (pushover) to study the behaviour of the structure under combined seismic action. In addition to these global approaches, local analyses were conducted to assess specific collapse mechanisms, using a kinematic model. These mechanisms were identified as critical, following a survey campaign, coupled with on-site inspections and a diagnostic campaign, providing a detailed understanding of the building structural configuration. This study adheres closely to the Italian guidelines for the heritage protection and conservation ensuring the structural safety in harmony with historical and architectural value. The findings of this research have identified the structural condition of the Church, ensuring long-term preservation of its structure and artistic treasures.