In this chapter, we explore key techniques that are intertwined with single-molecule imaging but may not be directly linked. Nonetheless, these techniques are key to understanding the behavior and characteristics of single molecules in living cells. Although visiting all methods is not possible and may not be in the scope of this book, we covered a few widely used ones. The techniques include single-molecule tracking and widely used single-molecule force spectroscopy techniques (Optical tweezers and Atomic force microscopy). In addition, we discussed a recent advance in SMLM, which is purely based on events, i.e., change in photon emission from single molecules. This has an advantage over traditional SMLM, specifically, with the event-based SMLM capable of high-density localization, large PAR-shift, high spatio-temporal resolution, and volume imaging. Finally, the chapter summarizes potential applications that were thought impossible a decade before, including protein folding and molecular kinetics in a live cell.