Dionysius of Fourna was a Greek hieromonk and icon painter who compiled the renowned “Hermeneia” painting manual (ca. 1730 AD). This text comprises of two parts, the first dealing with merely technical information and the second with iconographical dictations. The technical part contains numerous recipes that pertain to most of the practical aspects of painting, and includes several pigment manufacturing recipes. Among the latter, two recipes for the manufacturing of lake-type pigments are included; the first describes the preparation of a lake using dye extracted from kermes, while the second dictates the extraction of dyes from cloth parts/shearings and the precipitation of corresponding lake pigments. In the current study the components mentioned in the Dionysius’ recipes were identified and attempts were made to spot the pertinent raw materials. Ultimately it was impossible to spot and collect raw kermes, therefore cochineal was used instead. Reproduction attempts returned colored substances which were thoroughly studied through SEM-EDX. Subsequently, the products were mixed with selected binders, applied on gessoed wooden panels and studied through colorimetry. Results reveal that the Dionysius’ “Hermeneia” recipes are indeed applicable and yield usable lake pigments, and suggest that the choice of binder may affect considerably the appearance of the corresponding paints.