The adaptive capacity of university buildings is fundamental to prolonging their service life in an efficient and sustainable manner, ensuring that the needs of users are adequately met and contributing to quality in teaching. However, it is possible to assess the performance of buildings, taking into account technical performance features as well as the expectations of their users, making it possible to make adjustments that make buildings more resilient and sustainable. Thus, this article aims to present the development of methodological procedures and standardized Post-Occupancy Evaluation (POE) tools applied to the university context, focusing on the adaptability of buildings and the improvement of environmental, technical, and functional performance throughout their life cycle. This article presents the propositional research stage and part of the empirical research of the ongoing master’s thesis entitled “Adaptability in University Buildings: Diagnoses and Strategies from Post-Occupancy Evaluation”. The study is based on the hypothesis that systematic, specific, and adaptability-oriented POE can identify weaknesses and potentialities in buildings, contributing to more effective and sustainable interventions. The results of the initial application in the case study of a university building at the Federal University of Uberlândia are discussed, using the Walkthrough tool. The focus is on the adaptive capacity of the built environment, contributing to more efficient and sustainable management of academic spaces. Post-occupancy evaluation (POE) is used for continuous assessments on buildings’ performances, taking into account the views of experts and users. This approach provides a broad view of the real and current situation of the building space, providing important information and data for the efficient operation and maintenance of buildings, mitigating environmental impacts, reducing waste and optimizing resources throughout the building’s life cycle. It directly aligns with the Sustainable Development Goals, especially SDG 11, promoting resilient, sustainable and more inclusive communities. As a result, the research led to the creation of standardized Post-Occupancy Evaluation (POE) tools capable of identifying impacts and measuring the adaptive capacity of buildings. The application of the method, with emphasis on walkthrough, allowed for the validation of the tools and the collection of relevant data for space management. It was found that continuous assessments support more efficient maintenance and planning decisions, promoting sustainable and adaptable environments. The integration between technical and administrative aspects and user perception reinforces the importance of participatory and evidence-based practices in university estate management.