Relationships are the fundamental building block of organizations, yet the field lacks a validated and comprehensive measure of the relational climate in an organization. In this chapter, we develop a conceptual definition of relational climate, operationalized as the degree of shared vision, compassion, and relational energy in a given environment. In Study 1, we develop an item pool consisting of 51 items and then use a Q-sort procedure to assess content validity. In Study 2 the item pool is further reduced using exploratory factor analysis. This is followed by a confirmatory factor analysis that finds initial support for the three-dimension structure of relational climate. Study 3 provides further evidence of convergent and discriminant validity and assesses the criterion validity of the construct in relation to LMSX, perceived organizational support, and procedural justice. Finally, in Study 4, the factor structure of relational climate is successfully replicated and criterion validity is further assessed in relation to instrumental ethical climate and affective organizational commitment. Implications for theory, limitations, and future research are discussed.