Wound healing is a highly dynamic and regulated biological process that is essential for restoring tissue integrity and function following an injury. It progresses through four overlapping phases: hemostasis, inflammation, proliferation, and remodeling, all coordinated by complex cellular and molecular interactions. Key cellular players such as keratinocytes, fibroblasts, endothelial cells, and immune cells communicate through cytokines, chemokines, and growth factors, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and transforming growth factor-beta (TGF-β), to facilitate tissue repair. While acute wounds follow a predictable healing trajectory, chronic wounds often stall due to ongoing inflammation, impaired angiogenesis, and extracellular matrix (ECM) dysfunction, frequently exacerbated by diabetes, vascular diseases, and infections. This chapter explores the cellular and molecular mechanisms of wound healing, focusing on key signaling pathways, immune regulation, and therapeutic interventions. Emerging strategies, including stem cell therapy, bioengineered scaffolds, and gene editing, offer promising solutions for accelerating wound repair. Advances in nanotechnology and smart biomaterials enhance drug delivery and wound monitoring, while artificial intelligence (AI) enables personalized treatment approaches. By integrating regenerative medicine, bioengineering, and precision therapeutics, innovative wound healing strategies can revolutionize chronic wound management, ultimately improving clinical outcomes and patient quality of life.