The paper aims to comprehensively investigate the correlation between ‘culture’ and ‘urban spatial structure’ within the context of the Cultural Capital of Europe. Four cities, which were designated as European Capitals of Culture (ECoCs), were examined: Athens (1985), Glasgow (1990), Lisbon (1994), and Thessaloniki (1997). An extension of the Space Syntax theory is proposed to develop an epistemologically reliable tool for investigating the newly introduced concepts of ‘Urban Spatial Identity’ and ‘Eurocal Cultural-and-Spatial Identity’. This process results in constructing a set of principles suitable for guiding the programming of spatial interventions by candidate ECoCs, ensuring compliance with EU criteria and potentially establishing a ‘new concept paradigm’ within the ECoC Action. Through this process, candidate cities can leverage their uniqueness to create meaningful urban environments by blending their local and European spatial identities, thus optimizing and enhancing their ECoC legacy.