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The recent development of holographic tomography in flow cytometry enables to bridge the gap with fluorescence microscopy by adding computational intracellular specificity to single-cells in 3D and stain-free mode. In this paper we report the main advances in this topic, focusing on our recent results and providing perspective studies in real biomedical fields.

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Advances in Stain-Free 3D Imaging Flow Cytometry by High-Content Holographic Tomography

  • 会议论文

  • 发表日期：

    2026年

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  • Pasquale Memmolo,
  • Daniele Pirone,
  • Giusy Giugliano,
  • Francesca Borrelli,
  • Marika Valentino,
  • Vittorio Bianco,
  • Lisa Miccio,
  • Pietro Ferraro

摘要

The recent development of holographic tomography in flow cytometry enables to bridge the gap with fluorescence microscopy by adding computational intracellular specificity to single-cells in 3D and stain-free mode. In this paper we report the main advances in this topic, focusing on our recent results and providing perspective studies in real biomedical fields.