Among the organic pollutants, polyaromatic hydrocarbons are one of the utmost widely distributed in soil, water, and biological systems. The poly aromatic hydrocarbons comprise several aromatic fused rings and are made due to the partial burning of carbon-based matter such as fossil fuels, biomass, food processing, and industrial emissions. As they are easily accumulated in the environment leading to serious ecological and health risks. Long-term exposure to PAH be associated with various health issues like cardiovascular complications, carcinogenicity, and development disorders. Therefore, effective monitoring and mitigation strategies for their detection are very crucial mandates. The conventional techniques for the PAHs include GC, CE, HPLC, and SERS. Despite high sensitivity and specificity, these methods are expensive and need laborious sample preparations leading to the dependency on controlled laboratory conditions. In contrast, sensors and biosensors provide a promising alternative to detect PAHs selectively and quickly. The current chapter represents the recent developments and challenges of sensor and biosensor-based approaches for PAH detection.