The present study focused on evaluating the influence of 43 and 53 grade ordinary Portland cement on the performance of prestressed concrete beams under impact loading. The mix was prepared based on the 43 and 53 grade cement considering the grade of M45 concrete. The study is focused on designing prestressed concrete girders for railway bridges, spanning 15.4 m. A reduced scale beam of 1540x150x150 mm was prepared incorporating 3-ply 3 mm prestressing strands of four numbers. Also, the top and bottom reinforcements are 2 numbers of 6 mm confined with 6 mm stirrups of 90 mm spacing. These beams underwent impact tests having a mass of 294 kg dropped from a height of 0.5m and the key parameters such as peak impact load, reaction force, displacement and deformed profiles are studied. It is concluded that the cement grade has a noticeable influence on the performance of reinforced concrete beams, however it is less pronounced in prestressed beams.