Bladder organoids have emerged as promising three-dimensional (3D) in vitro models that faithfully recapitulate the structural and functional characteristics of human bladder tissue. Derived from primary urothelial cells, stem cells, or patient tumor samples, these organoids provide a physiologically relevant platform for studying both healthy bladder biology and disease progression, including cancer, infection, and inflammatory conditions. By maintaining key features such as cellular heterogeneity, tissue polarity, and urothelial differentiation, bladder organoids enable the investigation of normal tissue regeneration, response to injury, and molecular mechanisms underlying pathological states. Moreover, disease-specific bladder organoids, particularly those derived from patients with bladder cancer, offer valuable tools for drug screening, biomarker discovery, and precision medicine applications. This review highlights recent advances in bladder organoid culture systems, their applications in modeling bladder disorders, and ongoing efforts to integrate them into translational research and personalized therapeutic strategies.