High demand in the last years for products with specific characterization has led to the development and advancement of materials. Carbon fibre reinforced polymer (CFRP) is one of the advanced composite materials used. The development in materials has continued by using additive manufacturing of CFRP in different applications. Studying the characterization of CFRP manufactured in additive manufacturing is important to understand its impact. Therefore, this study focuses on the characterization of the fibre-matrix interface in 3D-printed continuous carbon fibre-reinforced thermoplastic composites (CFRTPCs) using Nano-indentation techniques. The performance of composite materials is highly dependent on the quality of the fibre-matrix interface. In 3D printed composites, defects such as voids and poor interfacial bonding, inherent to the layer-by-layer deposition process, can play a vital role in mechanical properties. The study employs Nano-indentation to characterize the micromechanical properties of the fiber-matrix interface in 3D-printed continuous carbon fiber-reinforced polymer composites. Results are compared with compression-moulded specimens to evaluate manufacturing-induced variations. Findings reveal that interface properties are strongly influenced by fibre volume fraction, voids, and processing parameters. Optimization of 3D printing parameters, coupled with compression moulding, is shown to enhance interfacial adhesion, thereby improving mechanical performance. The findings suggest that optimization of 3D printing parameters and the use of compression moulding can significantly improve interfacial adhesion, leading to enhanced mechanical performance of the composites.