Effective Degradation of Biosynthesis Low Density Polyethylene (LDPE-Gelatin) Film
摘要
The accumulation of plastic waste has recently been recognized as a critical environmental challenge, impacting all life forms, natural ecosystems, and economies worldwide. Currently, biodegradable plastic made from biopolymers or bio-based polymers is becoming increasingly widespread in the replacement of synthetic plastic. However, there is a lack of knowledge about the degradation of plastic with gelatin. Therefore, highly versatile Pseudomonas aeruginosa (IES-1) bacteria isolated from the coastal area of Clifton Karachi, Pakistan, were analyzed by 16 s rDNA sequence analysis for degradation assessment. A combination of scanning electron microscopy and FT-IR spectroscopy was used to detect surface and chemical changes in LDPE films caused by bacteria. The isolated bacteria were competent to degrade LDPE-gelatin by 70 ± 0.8% gravimetric weight over 8 weeks. Also, there is a significant increase in tensile strength has been recorded by adding LDPE with gelatin (0.8% gelatin-LDPE film 56 ± 1.15 MPa), whereas, pure gelatin bioplastic film exhibited tensile strength, at 6.3 ± 0.20 MPa which suggests that gelatin accelerates the process of degradation in LDPE films.