This study aimed to provide a comprehensive data analysis of airspora present in different cities of Sindh. It focused on identifying major allergenic pollen. The research was conducted in three major cities of Sindh: Karachi, Hyderabad (Tandojam), and Khairpur. Burkard’s 7-Day recording volumetric Spore Trap was used for data collection. Major pollen types reported from these cities were Amaranthaceae, Poaceae (Grass pollen), Asteraceae, Cyperus sp. (Cyperaceae), Eucalyptus globulus, Prosopis juliflora, Acacia/Albizia type, Tamarix indica, Guaiacum officinale, Leucaena leucocephala, Typha angustifolia¸ Rumex dentatus, Syzygium cumini, Parkinsonia aculeata, Mangifera indica, Moringa oleifera etc. Seasonal variations were observed, with the highest pollen counts recorded between April and September, while the lowest were detected in December and January. Present findings may contribute a significant role to a better understanding of airborne allergens, aiding in accurate diagnosis and management of pollen-induced allergies. While, in winters lowest pollen count were observed from December to January. These aerobiological surveys are valuable for the accurate diagnosis of allergenic pollen types prevalent in specific geographical regions.