Stevia rebaudiana is known as natural sweetest shrub that contains steviol glycosides which are non-toxic and with zero calories. Salinity stress significantly inhibit plant growth by causing osmotic stress and oxidative damage. Nitrogen is crucial for plant development and photosynthetic pigments. A pot experiment was carried out to examine the impact of nitrogen on photosynthetic pigments and antioxidant activities of Stevia under salinity stress. Nitrogen fertilizer was used in the form of urea. Seeds of Stevia were collected from Ayub Agricultural Research Institute, Faisalabad. Two salinity (0, 100 mM NaCl) levels and four nitrogen treatment levels (0, 0.1, 0.2, 0.3 g N kg−1 soil) were applied to analyse the impact on physiological parameters. Three replications of this pot trial were conducted using a completely randomized design (CRD). The results showed that nitrogen (0.2 g N kg−1 soil) considerably reduced H2O2 (P < 0.001) and MDA (P < 0.001) while increased carotenoids (P < 0.01), chlorophyll a (P < 0.001), b (P < 0.01), and a/b (P < 0.05) and antioxidants activity (CAT (P < 0.01), SOD (P < 0.001) and POD (P < 0.01)). Therefore, moderate nitrogen application can enhance photosynthetic pigments and antioxidant activities, alleviating the salt induced-toxicity in Stevia. This study could recommend low dose of nitrogen (0.2 g N kg−1 soil) utilization in the field to combat salinity-induced effects in plants.