Microbial biofilms pose a major challenge to the food industry, as they can contain persistent sources of contamination that lead to food spoilage or the transmission of microbial diseases. Microorganisms that form biofilms demonstrate significantly greater resistance to disinfectants and biocides than their planktonic forms. Therefore, the antimicrobial efficacy of many sanitizers tends to be reduced when confronted with foodborne pathogens. The search for antimicrobial and antibiofilm alternatives is essential for controlling pathogenic microorganisms. Nanomaterials such as silver, copper, zinc, titanium, and selenium stand out for their effectiveness and are used in food preservation and surface decontamination. These structures could be applied in different surfaces to extend the contribution of safety foods. However, their use requires caution, as they can pose health and environmental risks. This chapter explores the use of antimicrobial nanoparticles to prevent biofilm formation on food processing surfaces.