Scholarlink

This chapter provides a comprehensive overview of cutting-edge methodologies for the precise localization and quantification of Azospirillum in plant-soil ecosystems. Accurate tracking of Azospirillum populations is pivotal for evaluating the efficacy of these plant growth-promoting rhizobacteria (PGPR) as biological inoculants and assessing their ecological impact on indigenous microbial communities. The chapter systematically examines three major methodological frameworks: (i) reporter gene-based systems (lacZ, gusA, and gfp); (ii) immunological techniques (ELISA and immunofluorescence); and (iii) nucleic acid-based methods, emphasizing quantitative PCR (qPCR) and fluorescence in situ hybridization (FISH). Additionally, emerging technologies are explored, including DNA biosensors, flow cytometry, and synthetic microbial communities (SynComs), offering new perspectives for monitoring Azospirillum dynamics in complex soilSoil-plant systems. Future directions emphasize the integration of next-generation sequencing with modular bacterial tags (MoBacTag) for enhanced strain-level tracking capabilities. A key highlight is the presentation of a validated gold standard protocol for Azospirillum tracking through qPCR, providing researchers with standardized procedures for reliable bacterial enumeration across environmental samples, from rhizosphere to plant endosphere compartments.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Tracking and Quantification of Azospirillum spp.

  • 章节

  • 发表日期：

    2025年

查看全文
  • Willian Y. Takahashi,
  • Carolina W. Galvão,
  • Anton Hartmann,
  • Anahi Coniglio,
  • Fabricio Dario Cassán,
  • Rafael M. Etto

摘要

This chapter provides a comprehensive overview of cutting-edge methodologies for the precise localization and quantification of Azospirillum in plant-soil ecosystems. Accurate tracking of Azospirillum populations is pivotal for evaluating the efficacy of these plant growth-promoting rhizobacteria (PGPR) as biological inoculants and assessing their ecological impact on indigenous microbial communities. The chapter systematically examines three major methodological frameworks: (i) reporter gene-based systems (lacZ, gusA, and gfp); (ii) immunological techniques (ELISA and immunofluorescence); and (iii) nucleic acid-based methods, emphasizing quantitative PCR (qPCR) and fluorescence in situ hybridization (FISH). Additionally, emerging technologies are explored, including DNA biosensors, flow cytometry, and synthetic microbial communities (SynComs), offering new perspectives for monitoring Azospirillum dynamics in complex soilSoil-plant systems. Future directions emphasize the integration of next-generation sequencing with modular bacterial tags (MoBacTag) for enhanced strain-level tracking capabilities. A key highlight is the presentation of a validated gold standard protocol for Azospirillum tracking through qPCR, providing researchers with standardized procedures for reliable bacterial enumeration across environmental samples, from rhizosphere to plant endosphere compartments.