DevOps is a collaborative approach integrating development and operations, emphasizing agility and efficiency in software delivery. By breaking down traditional silos and promoting continuous collaboration, DevOps aims to streamline workflows and enhance product quality. While DevOps promises many benefits, it can also be challenging to adopt it in an organization, especially in terms of cultural change. This study focuses on the cultural challenges that organizations are facing while adopting DevOps. This research employs a dual-phase methodology to address the cultural challenges of DevOps adoption. First, a Systematic Literature Review follows established guidelines to collect insights from a wide range of reputable sources. The second phase involves a collaborative Focus Group, where academic expertise and industry perspectives converge. Through iterative discussions, the best identified practices are accurately mapped to corresponding cultural challenges. The systematic examination of 22 selected studies through the Systematic Literature Review resulted in the identification of 19 prevalent cultural challenges and 23 best practices for DevOps adoption. A Focus Group facilitated the careful mapping of these best practices to their corresponding cultural challenges. The results provide a concrete and applicable solution for organizations seeking to overcome cultural challenges, ensuring a smoother and more effective transition to DevOps principles in diverse operational settings.