Skeletal age estimation is an important method used in paediatrics, orthodontics, endocrinology, and forensic science to evaluate biological maturation. The current chapter scrutinizes key procedures for estimating skeletal age, and the cervical vertebral maturation index (CVMI), skull-based assessments, dental development analysis, and hand-wrist radiographs. Every method adds acumens into skeletal maturity using markers like ossification, tooth eruption, and suture closure including scoring systems similar to the Tanner-Whitehouse procedure and Fishman Skeletal Maturity Indicators providing standardised age evaluation. The current chapter also examines anatomical changes related to age, such as the shifting position of the mental foramen, variations in bone density, and the narrowing of the gonial angle. The connection between dental and skeletal age is discussed, displaying the significance of utilizing multiple evaluation processes to account for each variation because of nutrition, environmental factors, and genetics. These methods are important for identifying and determining age in forensic cases, planning orthodontic treatments, and growth disorders.