Research in analytical chemistry is mainly based on the working principle of the system and its parameters. This research aimed to advance the analytical devices for milk oxytocin detection by implementing fabrication methods and gaining insight into the qualitative assessment of oxytocin, aiding cost-effective, user-friendly, and high-sensitivity qualitative colorimetric detection based on paper-microfluidics principles and parameters by creating a real-time milk assessment kit. Over the years, significant advancements and innovations have been reported in theragnostic approaches, leading to improved precautionary and clinical methods. This work introduces a device detecting oxytocin in milk using gold nanoparticles, a paper-microfluidics device, mainly a lateral flow assay immobilized with testing reagents and enclosed in an outer case, and the detection analyte primarily based on the colorimetric detection resulting in blue coloured reaction product and the intensity of colour varies with the concentration of analyte in sample. And, the study was validated with calculated repeatability percentage and concluded by evaluating the detection limit of different concentrations of the analyte. By highlighting its implementations and next-generation advancements in analytical chemistry, limitations and challenges have been observed that need to be addressed to develop another extension in analytical chemistry with a good incorporation of synthetic biomimetic platforms into biomedical research. Thus, this work may offer different paths to developing user-friendly biomedical devices of different specificities, but that rely on the same working principles.